This article examines the influence of the pre-service training on mentor teachers’ role perception in the Professional Development Schools (PDS). The perceptions of the mentor teachers were examined regarding the influence of their teacher education as student teachers on their role perception in the PDS, and whether a difference exists between the perceptions of mentor teachers who were trained to teach via different approaches. The opinions of the pedagogical instructors were also examined, regarding their opinion on the difference between the role performance of Locals and Newcomers. The findings reinforce the fact that preparing mentor teachers is important, especially, if they mentor in a different context from that with which they were once familiar when students themselves.